Nikon SMZ25

Nikon’s SMZ25 stereomicroscope combines macro and micro imaging in one instrument for convenient viewing and manipulation of single cells to whole organisms. The stereomicroscope is equipped with two cameras allowing acquisition of both fluorescence and bright field pictures.

There are 4 filter sets for Epi-florescence:

DAPI, GFP, CY3, and CY5.

We installed a stage that allows the capturing of large images, covering 150mm x100 mm dishes (for example 96 well, 24 well, 6 well dishes).

There is a micromanipulation station adjacent to the stereomicroscope allowing the injection of dyes/reagents and following their location/influence on the specimen.

 

SMZ25 configuration

 

Hamamatsu ORCA-Flash 4.0

Prior Proscan III motorized stage

Intensilight C-HGFIE fluorescence light source

NIS-Elements image acquisition software

Filters:

Illumination Setting

Excitation          

Dichroic          

Emission

ET-BFP                        

ET405/20x        

T425lpxr         

ET460/50m

ET-EGFP (FITC/Cy2)   

ET470/40x        

T4951pxr

ET525/50m

ET - mCherry, Texas Red       

ET560/40x

T585lpxr        

ET630/75m

ET - Cy5 Longpass

ET620/60x

T660lpxrxt

ET665lp

 

Objectives:

Magnification

N.A.

Working distance

Type

0.5 x

NA: 0.078

W.D.: 71mm

P2-SHR Plan Apo

1X   

NA: 0.15                                        

W.D.: 60mm

P2-SHR Plan Apo

 

   
Nikon SMZ25

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