The N-SIM S Super Resolution (SR) Microscope is a unique high-speed structured illumination system for multi-dimensional imaging for both live and fixed cells.

The platform achieves acquisition speeds of up to 15 fps enabling super-resolution time-lapse imaging of live cells and intracellular dynamics at twice the spatial resolution of conventional light microscopes (up to 115nm in XY).

SIM requires low illumination intensities, and can be used with any fluorophore, which renders as very gentle and useful SR technique especially for live sub-cellular imaging.

The system supports various observation modes:

The 2D-SIM mode/TIRF-SIM mode capturing super-resolution 2D images at high speed with incredible contrast. The TIRF-SIM mode enables Total Internal Reflection Fluorescence observation at double the resolution of conventional TIRF microscopes, facilitating a greater understanding of molecular interactions at the cell surface.

The 3D-SIM mode generates structured illumination patterns in three dimensions to deliver a two-fold improvement in lateral and axial resolution. Two reconstruction methods (“slice” and “stack”) are available to optimize results for application requirements (e.g. sample thickness, speed, etc.).

N-SIM-S supports different types of objectives (Air/Oil/Silicon/Water Immersion) in various magnifications (40x,60x & 100x) and allows imaging high FOV, up to 110µm x 110µm (Actual view field on sample, using 40x or 60x air objectives).

The system includes four laser lines (405nm, 488nm, 561nm & 640nm).