Nikon Fluorescent

Automated Inverted Microscope w/ Perfect Focus & 37°C Incubation Chamber (Fluorescence microscope)

Imaging modalities with standard configuration:
• Phase contrast
• DIC
• Fluorescence
• Live cell imaging - long term
• Color imaging
• Nikon Ti motorized inverted microscope with DIC, phase, and epi-fluorescence optics and Perfect Focus System
• Photometrics Prime 95B camera
• Nikon DS-Fi2 color CCD camera
• Prior Proscan III motorized stage
• Prior LumenPro200 fluorescence light source
• NIS-Elements image acquisition software
• Custom built 37° C, 5% CO2, humidity control Incubation chamber Okolab

Filters:

Illumination Setting	Excitation	Dichroic	Emission
DAPI	350/50	400LP	460/50
EGFP/FITC/CY2	470/40	T4951pxr	525/50
CY3/TRITCP	545/25	5651pxr	605/70
CY5	620/60	T660lpxr	700/75

Objectives:

Magnification	N.A. Immersion	Type
4 x	0.13 dry	PlanFluor	PhL
10 x	0.3	PlanFluor
20 x	0.45 dry	PlanApro	DIC
20 x	0.45	PlanApro	Ph
40 x	0.75 dry	PlanFluor
60 x	1.3 oil	PlanFluor	DIC
60 x	1.4 oil	PlanApro	DIC
100x	1.4 oil	PlanApro	DIC