Nikon Fluorescent

Automated Inverted Microscope w/ Perfect Focus & 37°C Incubation Chamber (Fluorescence microscope)

 

Imaging modalities with standard configuration:
• Phase contrast
• DIC
• Fluorescence
• Live cell imaging - long term
• Color imaging
• Nikon Ti motorized inverted microscope with DIC, phase, and epi-fluorescence optics and Perfect Focus System
• Photometrics Prime 95B camera
• Nikon DS-Fi2 color CCD camera 
• Prior Proscan III motorized stage
• Prior LumenPro200 fluorescence light source
• NIS-Elements image acquisition software
• Custom built 37° C, 5% CO2, humidity control Incubation chamber Okolab

 

Filters:

Illumination Setting     Excitation       Dichroic      Emission
DAPI                 350/50    400LP     460/50
EGFP/FITC/CY2        470/40            T4951pxr          525/50
CY3/TRITCP           545/25         5651pxr        605/70
CY5                                 620/60            T660lpxr      700/75

 

Objectives:

Magnification        N.A. Immersion          Type  
4 x                  0.13 dry          PlanFluor    PhL
10 x                 0.3                PlanFluor  
20 x                 0.45 dry           PlanApro      DIC
20 x                 0.45               PlanApro     Ph
40 x                 0.75 dry           PlanFluor  
60 x                 1.3 oil            PlanFluor   DIC
60 x                 1.4 oil            PlanApro    DIC
100x 1.4 oil     PlanApro  DIC
   
Nikon Fluorescent

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