Nikon Confocal A1R+

Nikon’s A1R+ confocal microscope enables capturing of high-quality confocal images at ultrahigh-speed and enhanced sensitivity. The A1R+ has a hybrid scanner head that incorporates both an ultrahigh-speed resonant scanner (up to 420 fps 512 x 32 pixel frames per second) and a high-resolution galvano scanner (from 64 x 64 up to 4096 x 4096 pixels). Simultaneous photo-activation and ultrafast imaging using these two scanners allow acquisition of rapid changes after photo-activation and enables observation of intermolecular interaction.

The A1R confocal is mounted on a fully automated inverted Nikon Ti2 microscope. A Nikon Perfect Focus System (PFS) is included that continuously determines the distance to the sample.

Life cell imaging is possible because the system is equipped with an on-stage incubation chamber controlling temperature, CO2-concentration and humidity.

The A1R confocal is equipped with four standart fluorescence detectors (PMT) and has two high-sensitive GaAsP-detectors for standard and spectral imaging.

Nikon A1R+ Specifications:

Laser unit LU-N4S (4-laser unit)

405 nm, 488 nm, 561nm, 640nm.

Four lasers are installed.

Standard fluorescence detector

400-750 nm, Detector Unit: 4 standard PMTs

Spectral Detector: 2 GaAsP

Scan head (Standard image acquisition)

Scanner: Galvano scanner x2 Pixel size: max. 4096 x 4096 pixels Scanning speed: Standard mode: 2 fps (512 x 512 pixels, bi-direction), 24 fps (512 x 32 pixels, bi- direction) Fast mode: 10 fps (512 x 512 pixels, bi-direction), 130 fps (512 x 32 pixels, bi-direction)*2

Scan head (High-speed image acquisition)

Scanner: Resonant scanner (X-axis, resonance frequency 7.8 KHz), Galvano scanner (Y-axis) Pixel size: max. 512 x 512 pixels Scanning speed: 30 fps (512 x 512 pixels) to 420 fps (512 x 32 pixels), 15,600 lines/sec (line speed) Zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x)

Pinhole

12-256 µm variable

Spectral detector A1-DUVB GaAsP detector unit

Number of channels: 1 GaAsP detector with variable emission plus 1 optional, fixed GaAsp detector with a user-defined dichroic mirror and barrier filter .
Wavelength detection range: 400 - 720 nm, narrowest: 10nm, broadest:320nm
Maximum pixel size: 4096 x 4096 (CB mode/VB mode)
Wavelength resolution: 10 nm, wavelength range variable in 1 nm steps
Compatible with Galvano and Resonant scanner.

Z step

Motorized

Microscope Body

ECLIPSE Ti-E inverted microscope, Motorized XY stage.

Objectives

Magnification	N.A.	Immersion	Type
10X	0.45	dry	Plan-Apochromat
20 x MI	0.75	oil, glycerin, Water	Plan-fluor
40X	1.3	oil	Plan-fluor
60X	1.4	oil	Plan-Apochromat
100X	1.4	oil	Plan-Apochromat