Nikon Confocal A1R+

 

Nikon’s A1R+ confocal microscope enables capturing of high-quality confocal images at ultrahigh-speed and enhanced sensitivity. The A1R+ has a hybrid scanner head that incorporates both an ultrahigh-speed resonant scanner (up to 420 fps 512 x 32 pixel frames per second) and a high-resolution galvano scanner (from 64 x 64 up to 4096 x 4096 pixels). Simultaneous photo-activation and ultrafast imaging using these two scanners allow acquisition of rapid changes after photo-activation and enables observation of intermolecular interaction.

The A1R confocal is mounted on a fully automated inverted Nikon Ti2 microscope. A Nikon Perfect Focus System (PFS) is included that continuously determines the distance to the sample.

Life cell imaging is possible because the system is equipped with an on-stage incubation chamber controlling temperature, CO2-concentration and humidity.

The A1R confocal is equipped with four standart fluorescence detectors (PMT) and has two high-sensitive GaAsP-detectors for standard and spectral imaging.

Nikon A1R+ Specifications:

 Laser unit LU-N4S  (4-laser   unit)

 405 nm, 488 nm, 561nm, 640nm. 

 Four lasers are installed.

 Standard fluorescence detector

 400-750 nm, Detector Unit: 4 standard  PMTs

 Spectral Detector: 2 GaAsP

 Scan head (Standard image   acquisition)

 Scanner: Galvano scanner x2 Pixel size:   max. 4096 x 4096 pixels   Scanning speed:  Standard mode: 2 fps (512 x 512 pixels, bi-direction), 24 fps (512 x 32 pixels, bi-  direction) Fast mode: 10 fps (512 x 512 pixels, bi-direction), 130 fps (512 x 32 pixels, bi-direction)*2 

 Scan head  (High-speed image   acquisition)

 Scanner: Resonant scanner (X-axis,   resonance frequency 7.8 KHz), Galvano   scanner (Y-axis) Pixel size: max. 512 x   512 pixels Scanning speed: 30 fps   (512 x   512 pixels) to 420 fps (512 x 32 pixels),  15,600 lines/sec (line speed) Zoom: 7   steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x) 

 Pinhole

 12-256 µm variable

 Spectral detector A1-DUVB   GaAsP detector unit

Number of channels: 1 GaAsP detector with variable emission plus 1 optional, fixed GaAsp detector with a user-defined dichroic mirror and barrier filter .
Wavelength detection range: 400 - 720 nm, narrowest: 10nm, broadest:320nm 
Maximum pixel size: 4096 x 4096 (CB mode/VB mode) 
Wavelength resolution: 10 nm, wavelength range variable in 1 nm steps 
Compatible with Galvano and Resonant scanner.

 Z step

 Motorized

 Microscope Body

 ECLIPSE Ti-E inverted microscope,  Motorized  XY stage.

 Objectives

 

Magnification

N.A.

Immersion

Type

10X

0.45

dry

Plan-Apochromat

20 x MI

0.75

oil, glycerin, Water

Plan-fluor

40X

1.3

oil

Plan-fluor

60X

1.4

oil

Plan-Apochromat

100X 1.4 oil Plan-Apochromat

 

 Software

 NIS Elements: 2D analysis, 3D volume, spectral unmixing, Deconvolution.

 FRAP, FLIP, FRET (option), photoactivation, co-localization.

 

 

   

 
Nikon Confocal    Robert Family

The instrument is available for trained users in a self-operated mode.

Untrained users must contact core personnel to schedule hands-on training.

For ordering this equipment use our booking system.

 


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