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Cytation 5 | Core Research Facilities

Cytation 5

Cytation 5 combines automated digital microscopy and conventional microplate detection in a configurable, upgradable platform for cell-based assays that cannot be performed on a standard plate reader or microscope separately. This patented design, along with Agilent/Biotek Gen5 software, enables automated workflows across a vast range of biochemical and imaging applications. Information on cell morphology, signal localization, cell count and more is obtained quickly and easily exportable for data publication. Further, there is no longer need to process and analyze images one by one on a dedicated computer. The automated analysis function enables you to pre-program, begin the experiment and walk away.

 

Features:

 

  1. Image analysis common functions: cell count, confluence, cytoplasm analysis, intracellular analysis, subpopulation analysis, signal translocation, and much more.

  2. Image processing steps: stitching, Z-projection, deconvolution, digital phase contrast

  3. Imaging modes: fluorescence; phase contrast; high contrast brightfield; brightfield; color brightfield

  4. Plates: 6- to 384 well

  5. Incubation up to 650C and linear, orbital and double-orbital shaking

  6. Objectives: x4, x10, x20. X40

  7. Filters: Blue (DAPI), Green (GFP), Texas Red

  8. Imaging LED cubes: 365, 590

  9. Reading speed (kinetic): 96 wells 11 seconds; 384 wells: 22 seconds

 

 

         

 

cytation_5

 

 

 

 

 

This equipment is located in room 120 at Botnar building, Level -2.

If you want to use this equipment for the first time, please contact the person in charge.

 

 

 

                                          Back to main BSL2 lab

   

 

 

 

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