Novaseq 6000

The NovaSeq 6000 System leverages proven Illumina sequencing by synthesis (SBS) technology to deliver accurate data and robust performance. SBS chemistry uses reversible-terminator fluorescently labeled nucleotides. This method detects single bases as they are incorporated into growing DNA strands, reading billions of sequences in parallel.

Patterned flow cell technology

The NovaSeq 6000 System incorporates patterned flow cell technology to generate an unprecedented level of throughput for a broad range of sequencing applications. Patterned flow cells contain billions of nanowells at fixed locations, a design that provides even spacing of sequencing clusters. This delivers significant increases in sequencing reads and total output of the system.

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Flow Cell Type	Read Lengths (bp)	Expected Yield
SP	100, 200, 300, 500	800 million reads
S1	100, 200, 300	1.6 billion reads
S2	100, 200, 300	4 billion reads
S4	200, 300	10 billion reads