The Agilent 2100 Bioanalyzer system is an established automated electrophoresis solution for the sample quality control of biomolecules. The system integrates an instrument, data processing software, reagents, and a microfluidic chip specific for DNA, RNA, or protein analysis. It is suitable for next-generation sequencing (NGS), gene expression, biopharmaceutical, and genome editing workflows, delivering highly precise analytical evaluation of various sample types. Advantages of microfluidics-based automated electrophoresis over traditional gel electrophoresis include dramatically reduced sample (1 µL for nucleic acids, 4 µL for proteins) and reagent consumption, significantly faster analysis time, and less hands-on activities during sample preparation and data analysis. The Agilent 2100 Bioanalyzer system is ideal for the scientist requiring a versatile instrument for nucleic acids and protein electrophoresis analysis with moderately low throughput (10-12 samples) needs.

We don’t have kits in stock for the Bioanalyser. User should provide reagents, and we will instruct users on this equipment.

DNA

Bioanalyzer DNA kits provide microfluidic chips, reagents, and consumables needed to run DNA sample assays with your Agilent 2100 Bioanalyzer instrument. Kits are available in DNA and high sensitivity DNA versions, the latter providing increased sensitivity – down to 5 pg/µL for analyzing fragmented DNA or DNA libraries of limited abundance.

Perform highly accurate and precise DNA electrophoresis with the Bioanalyzer DNA analysis solutions. Together with the 2100 Bioanalyzer instrument, the DNA kits enable the analysis of next-generation sequencing libraries and high resolution multiplex PCR reactions, measuring precise size and concentration of all DNA fragments and smears. A large linear dynamic range allows detection of small impurities in PCR purifications.

Besides sample quality control of next-generation sequencing (NGS) libraries, additional research applications for the Bioanalyzer DNA assays include PCR amplicon analysis, multiplex PCR amplicons, gene expression analysis by RT-PCR, restriction fragment analysis, and the detection of targeted cleavage in gene editing studies.

Features:

• High Sensitivity from laser (LIF) detection of DNA fragments down to 0.1 ng.
• The broad linear dynamic range allows detection of weak bands without saturation from abundant fragments.
• Sizing and quantitation accuracy and reproducibility results from pre-packaged reagents, standardized assays and automated data analysis.
• Minimal sample consumption is required as only 1 µL is needed for the assay, saving precious material for downstream experiments.
• Fast results are provided, with the analysis of 12 samples performed in only 30 minutes (approximately).
• Safety is ensured with minimal exposure to hazardous materials, such as ethidium bromide, as only minimal reagents are used.

High Sensitivity DNA electrophoresis with the Bioanalyzer system enables improved quality control analysis of next-generation sequencing (NGS) library smears derived from just a few amplification cycles. Whether fragmented DNA or DNA sequencing libraries, the Bioanalyzer High Sensitivity DNA Kit reliably sizes and quantifies limited samples in the lower pg/µL concentration range.

The High Sensitivity DNA assays are often used for sample quality control for next-generation sequencing libraries. In addition, PCR amplicon analysis, multiplex PCR amplicons, gene expression analysis by RT-PCR, restriction fragment analysis, and detection of targeted cleavage in gene editing studies may all be carried out. Accuracy is ensured with a broad linear dynamic range and normalization to internal markers and ladder.

Features:

• Increased sensitivity for DNA analysis, down to 5 pg/µL for fragment analysis or 100 ng/uL for complex DNA samples, such as NGS libraries.
• A broad linear dynamic range that allows detection of less abundant PCR products.
• Accurate smear analysis within a specified size range of interest.
• Enhanced sizing accuracy, normalized to internal markers and external ladder.
• Quantitation accuracy and reproducibility calculated against precise internal standards.
• Minimal sample consumption is required as only 1 µL of sample is needed for analysis.
• Minimal exposure to hazardous materials, such as ethidium bromide, ensures safety.
• Achieve improved quality control analysis of next-generation sequencing (NGS) library smears derived from just a few amplification cycles.

RNA

Bioanalyzer RNA kits provide microfluidic chips, reagents, and consumables needed to run RNA sample assays with your Agilent 2100 Bioanalyzer instrument. Kits are available in RNA, high sensitivity RNA - providing increased sensitivity down to 50 pg of total RNA, and small RNA versions, the latter allowing you to resolve small nucleic acids in the size range of 6 to 150 nt.

The Bioanalyzer RNA 6000 Nano assay provides reliable and reproducible characterization of total RNA and mRNA from multiple sample types, from as low as 5ng of total RNA. The software will provide an objective measurement of RNA quality with RIN (RNA Integrity Number). Validated RIN values are available for eukaryotic, prokaryotic, and plant samples.

The Bioanalyzer RNA 6000 pico assay provides reliable and reproducible characterization of total RNA and mRNA from multiple sample types, from as little as 50 pg of total RNA. The software provides an objective measurement of RNA quality with RIN (RNA Integrity Number). Validated RIN values are available for eukaryotic, prokaryotic, and plant samples.

Features:

• Maximum flexibility is enabled with options to check total RNA, mRNA, or cRNA quality.
• Minimal sample consumption required with only need 50 pg of total RNA for an effective analysis.
• Easily determine the RNA integrity number (RIN) using in-built software that automatically assigns an integrity number to a total RNA sample. This tool was designed to provide scientists an objective measurement of degradation in RNA samples.
• RNase free consumables avoid sample degradation during analysis.
• Accurate results are achieved rapidly, with 11 samples analyzed in approximately 30 minutes.

The Bioanalyzer RNA kits allow dependable and precise integrity checks and sample quantitation prior to any RNA-dependent application. This ensures the data from downstream research processes, such as gene expression microarray, qPCR, and transcriptome sequencing, are valid and reliable.

Small RNA electrophoresis with the 2100 Bioanalyzer System allows separation and quantitation measurements of challenging RNAs, such as microRNA and exosomes, sized below 200 nt and are often present in limited abundance. The Bioanalyzer Small RNA assay provides a fast and sensitive analysis to resolve small nucleic acid samples in the size range of 6 to 150 nt.

In addition, the Bioanalyzer Small RNA assay is ideally suited for quality control of guide RNAs. In CRISPR/Cas workflows the synthesis of guide RNA (gRNA) is a key component for successful genome editing. gRNA created by in vitro transcription and purified may be run on the 2100 Bioanalyzer system and inspected for sizing, yield, impurities, and secondary structures.

Features:

• High resolution analysis, with a sizing range from 6 to 150 nucleotide, allows for separation of miRNA smears from tRNA.
• Minimal sample consumption. Only use as little as 50 pg of purified miRNA or 10 ng of total RNA for analysis in 1 µL volume.
• A faster and more sensitive alternative to agarose and polyacrylamide gels analysis for detection of small RNA.
• Compatibility with multiple sample types enables analysis of total RNA, fractionated, or synthetic samples.

For more information, please contact us at: genomics@ekmd.huji.ac.il