FACScan

 

This BD FACScan analyzer is a 5-parameter instrument for data acquisition. It has one laser at 488nm and can collect 3 flourescent and 2 scatter properties simultaneously. It is important to remember that only fluorochromes excited at ~488nm may be used on this instrument.  If you would like to use a fluorochrome with a different excitation wavelength, please consider using the LSRII or the LSRFortessa.

We train users to operate the flow cytometer and to use the software CellQuest.  Trained users are able to operate the instrument without supervision.  Facility staff is available to go over experiment design, discussion of data analysis or any other question users may have.

 

 

BD – FACScan Configuration

Laser

Parameter

Filter

Common dyes / fluorochromes

488nm

FL-1

530/30

FITC; GFP; CFSE; Alexa488

FL-2

585/42

PE; Alexa532; PI

FL-3

>670

PE-Cy5; PerCP; PI

 

 

   
FACScan

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