Digital PCR Bio-rad

 

The QX200 Droplet Digital PCR (ddPCR™) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications. The system provides an absolute measure of target DNA molecules with unrivaled performance in precision, accuracy, and sensitivity for quantitative PCR applications. The ddPCR system lets you detect rare DNA target copies with unmatched sensitivity, copy number variation with unrivaled accuracy and gene expression levels with the highest precision.

The QX200 droplet generator partitions samples into 20,000 nanoliter-sized droplets. After PCR on a thermal cycler, droplets from every sample are streamed in single file on the QX200 droplet reader. The PCR-posiitive and PCR-negative droplets are counted to provide absolute quantification of target DNA in digital form.

 

Benefits of the QX200 Droplet Digital PCR System:

  • Most precise and sensitive digital PCR solution for a wide variety of applications.
  • Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
  • Flexible assay setup — scalable for high sensitivity or high throughput.
  • Simple and easy-to-use workflow with 96-sample throughput.
  • Droplet partitioning by the QX200 Droplet Digital technology reduces bias from amplification efficiency and PCR inhibitors.

 

Applications and Uses:

• Copy number variation

• Rare sequence detection

• Mutation detection

• Gene expression analysis

• miRNA analysis

• Next generation sequencing sample quantification

 

 

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digital_pcr

For more information and primer design click here.

For more information, please contact us


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