BD LSR II

 

The BD LSR II analyzer is equipped with 4 laser:  near UV 375, Violet 405, Blue 488 and Red 633.

It is set up to detect up to 10 fluorescent parameters simultaneously.

The instrument is available for trained users in a self-operated mode. Untrained users must contact core personnel to schedule hands-on training.

Acquisition is done with BD FACSDiva™ software and FCS files should be exported after acquisition. Data analysis can be done with any third party analysis software. We have working stations with FlowJO and FCS-express softwares. We gladly train users to perform independent analysis and can assist with data translation.

Useful link:  BD Spectrum viewer

 

 

BD – LSR II  Configuration

Laser

BP

LP

Fluorophores / Fluorescent Proteins

Near UV (375nm)

530/30

505

 Indo-1 (Blue)

450/50

-

 DAPI; Hoechst; Hoechst 33258; CFP

Violet (405nm)

525/50

505

 BV510; AmCyan; V500; Alexa Fluor 430;   Live/Dead Fixable Dye Aqua; Qdot  525;   Pacific Green; Cascade Yellow

450/40

-

 BV421; Cascade Blue; Marina Blue; Pacific   Blue; efluor450; V450; AlexaFluor 405; Sytox   Blue

Blue (488nm)

780/60

735

 PE-Cy7

695/40

685

 PE-Cy5; PerCP; Percp-Cy5-5; 7-AAD

575/26

550

 DsRed; PE: PI

530/30

505

 FITC; Alexa Fluor488; GFP; CFSE;   Live/Dead Fixation Dye Green; Horizon   Brilliant Blue 515; Calcein Green (pH9);   Sytox Green

Red (633nm)

780/60

735

 APC-Cy7; APC-H7; APC-AlexaFluor750;   APC-eFluor780

660/20

-

 Alexa Fluor 647; APC; Cy5

 

 

   

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