The Nepagene electroporator is ideal for difficult-to-transfect cells such as primary cells, stem cells, immune cells, blood cells, etc.

The novel technology of 4-step pulse with voltage decay results in higher transfection efficiency and higher viability without need for special buffers.

1) Poring Pulse Mode:
This poring pulse is for forming pores (small holes) in cell membrane with minimum damage.

2) Polarity Exchanged Poring Pulse:
This can be applied to tissue transfection as well.

3) Transfer Pulse Mode:
This transfer pulse is for delivering the target molecules (DNA, RNA, etc.) into cells with minimum damage.

4) Polarity Exchanged Transfer Pulse:
This can increase the transfection efficiency.

NEPA21 Applications:

        In Vitro Transfection

• Into difficult-to-transfect cells such as primary cells, stem cells, etc.

• into any cell lines.

• with electroporation cuvettes. 

• with cell-culture-plate electrodes for cells in adherence .

        In Vivo Transfection

• Into mouse/rat brain, eye, muscle, skin, liver, kidney, testis, etc.

• with in-vivo electrodes .

        In Utero Transfection

• Into mouse/rat embryos: cerebral cortex, hippocampus, spinal cord, etc.

• with in-utero electrodes.

         In Ovo Transfection

• Into chick embryos: neural tube, limb bud, digestive organ, etc.

•  with in-ovo electrodes .

          Ex-Vivo Transfection

• Into explants, brain slices, organs, whole embryos, etc.

• with ex vivo electrodes.

Cuvettes for in vitro tranfection can be purchased at the unit.