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The FLUOstar Galaxy is a fully automated microplate based multi-detection reader which incorporates different measurement principles:
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Fluorescence.
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Fluorescence Intensity.
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Time-Resolved Fluorescence.
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Absorbance.
Applications:
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Ca2+ Measurements (e.g. Ca2+ Flux with Fura-2 or Indo-1).
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Enzyme Activity.
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Cell Toxicity.
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Cell, Proliferation and Viability.
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Immunoassays.
By integrating a high intensity xenon flash lamp, the Galaxy can read plate formats of 6-384 well plates in all measurement modes. Top-to-bottom readings.
Incubation
Temperature-regulated heating plates above and below the entire plate movement area provide uniform incubation up to 45°C.
Endpoint, slow and fast kinetic measurements
Kinetic applications can be defined, with interval times from 100 ms to several hours.
Multi-color detection
The fast filter wheels and the ability to define up to 8 sets of filter, each with individual gain and measurement timing parameters, allow analysis of multiple fluorophores in a single well (FRET, dual-emission, DNA-detection).
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FLUOstar Galaxy- Technical Specifications
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Measurement Principles
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Fluorescence
Fluorescence Intensity
Time-Resolved Fluorescence
Absorbance
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Light Source
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High-energy xenon flash lamp
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Detector
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Side window, current type photomultiplier tube
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Fluorescence Filters
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Excitation filters: 320, 490, 485, 544 nm.
Emission filters: 405, 460, 520, 590 nm.
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Absorbance Filters
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Filters: 405,450, 492, 590, 620 nm.
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Fluorescence Intensity
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Limit of detection: 0.9 fmol / well fluorescein
calculated according to the IUPAC standard: 3(SDblank)/slope
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Time-Resolved Fluorescence
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Limit of detection: 50 amol/well europium
calculated according to the IUPAC standard: 3(SDblank)/slope
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Microplate Formats
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up to 384 well and Fluorescence intensity and time-resolved fluorescence up to 1536 well plate.
Fluorescence black plate and Absorbance clear plate
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Reading Time
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Depends on test definition
shortest reading time: 15 s for 96-well plate
30s for 384 well plate 60s for 1536 well plate
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Incubation
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Incubation range from ambient +8 °C to 45 °C
in 0.1°C steps
Temperature stability 0.2 °C
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Shaking
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Orbital or linear shaking, user definable time and shake width
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