Metabolomics & Mass Spectrometry Equipment

The Metabolomics and Mass Spectrometry Laboratory is located in The Core Research Facility, Faculty of Medicine Ein Karem. The facility is dedicated to provide a comprehensive mass spectrometry services focussing on methods for Targeted and Untargeted Metabolomics.

Metabolomics is the comprehensive study of low molecular mass molecules (< 1500 Da) found in cells, tissue or biofluids of a living organism. It is a powerful tool to study metabolic processes, allowing identification of individual biomarkers or pattern signature that can accurately distinguish between certain states or conditions of an organism.

Metabolites are directly connected to the biochemical activity of cells in various tissues, thus, metabolomics study’s offers a powerful framework for analyzing the different phenotypes associated with physiological states at the molecular level.

The two major approaches in metabolomics consist of Targeted analysis - the determination and quantification of small set of known metabolites and Metabolite Profiling - the analysis of a large set of compounds, both known and unknown with respect to their chemical properties.

Our mission

The overall mission of the metabolomics facility is to support metabolism related projects by assisting throughout all stages of the research, starting from experimental design, sample preparation, data acquisition and analysis.

Metabolomics Platform

  • Our metabolomics research capabilities are based on Liquid Chromatography Mass Spectrometry (LC-MS) technology using high resolution accurate mass instrument (Q-Exactive Plus, Thermo Scientific) coupled with Ultra Performance Liquid Chromatography (UPLC) systems (Thermo scientific).
  • We are equipped with Electron Spray Ionization (ESI) and Atmospheric pressure chemical ionization (APCI) which allows the identification and quantitation of a broad range of compound classes such as (but not limited to):
  1. Carboxylic acids, amino acids
  2. Biogenic amines, polyamines
  3. Nucleotides, coenzymes and vitamins
  4. Mono- and disaccharides
  5. Fatty acids and steroids

 

      
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Metabolomics & Mass Spectrometry Services

The lab services includes:

  • Help in designing metabolomics experiments
  • Sample Preparation includes a variety of biological samples such as cell culture, biofluids and tissues.
  • LCMS data acquisition- Targeted and untargeted metabolomics as well as labeled isotopes tracing experiments
  • Method development tailored to specific projects
  • Data analysis using Compound DiscovererTM and Trace FinderTM.

 

Metabolomics & Mass Spectrometry Staff

02-6757572

ori.shalev@mail.huji.ac.il

02-6758462

alexandrae@savion.huji.ac.il